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Image Search Results
Journal: Cells
Article Title: Gestational Intermittent Hypoxia Enhances Mammary Stem Cells and Alters Tumor Phenotype in Adult Female Offspring
doi: 10.3390/cells13030249
Figure Lengend Snippet: Increased mammary stem and progenitor cell activity in GIH offspring rats. ( A ) Representative image of mammospheres. ( B ) Average primary and secondary mammospheres per well for 8-week-old GNx and GIH offspring rat mammary glands ( n = 4 rats/group). ( C ) Representative image of colonies. ( D ) Average mammary epithelial cell colonies per well ( n = 4 rats/group). ( E ) Average primary and secondary mammospheres per well for 20-week-old GNx and GIH offspring rat mammary glands ( n = 4 rats/group). ( F ) Average mammary epithelial cell colonies per well ( n = 4 rats/group). ( G ) Differences in gene expression in mammary epithelial cells isolated from mammary glands of GNx and GIH offspring quantified using qRT-PCR arrays ( n = three rats/group). ( H ) Representative images and quantification of phosphorylated SMAD2 (pSMAD2) staining in rat mammary ducts ( n = 5/group). Dotted lines indicate area of magnification for inset image. Magnification bar = 100 µm.
Article Snippet: Tissue sections were incubated with anti-rat Ki67 antibodies (1:250 dilution; Abcam, Waltham, MA, USA, ab157301),
Techniques: Activity Assay, Expressing, Isolation, Quantitative RT-PCR, Staining